Fabrication of oil-in-water high internal phase emulsions with enhanced antioxidative properties by modified starch/polyphenol mixtures: Effect of EGCG concentration, NaCl concentration, and temperature

Lidan Pan; Zimei Li; Jiayi Liu; Tenglong Geng; Xiaorui Liu; Die Dong; Li Guo; Chao Yuan; Bo Cui; Haiyan Liu

doi:10.1016/j.ijbiomac.2025.139585

Fabrication of oil-in-water high internal phase emulsions with enhanced antioxidative properties by modified starch/polyphenol mixtures: Effect of EGCG concentration, NaCl concentration, and temperature

Int J Biol Macromol. 2025 Jan 7:139585. doi: 10.1016/j.ijbiomac.2025.139585. Online ahead of print.

Authors

Lidan Pan¹, Zimei Li², Jiayi Liu², Tenglong Geng¹, Xiaorui Liu¹, Die Dong³, Li Guo⁴, Chao Yuan⁴, Bo Cui⁵, Haiyan Liu⁶

Affiliations

¹ State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China; School of Food Science and Engineering, Qilu University of Technology Shandong Academy of Sciences, Jinan 250353, China.
² School of Food Science and Engineering, Qilu University of Technology Shandong Academy of Sciences, Jinan 250353, China.
³ State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China; School of Food Science and Engineering, Qilu University of Technology Shandong Academy of Sciences, Jinan 250353, China. Electronic address: [email protected].
⁴ State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China.
⁵ State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China; School of Food Science and Engineering, Qilu University of Technology Shandong Academy of Sciences, Jinan 250353, China. Electronic address: [email protected].
⁶ Qingdao Bright Moon Seaweed Bio-Health Technology Group Co., Ltd, Qingdao 266400, China.

PMID: 39788252
DOI: 10.1016/j.ijbiomac.2025.139585

Abstract

In this study, oil-in-water (O/W) high internal phase emulsions (HIPEs) with enhanced antioxidative properties stabilized by octenyl succinic anhydride modified starch (OSAS)/(-)-Epigallocatechin-3-gallate (EGCG) mixtures were prepared. The influence of EGCG concentration (0-0.2 %, w/v), NaCl concentration (0-400 mmol/L), and temperature (25-90 °C) on the stability of the HIPEs was evaluated. The formation of O/W HIPEs, and OSAS/EGCG mixtures formed a thicker barrier on the surface of the oil droplets was confirmed using confocal laser scanning microscopy (CLSM) and cryo-scanning electron microscopy (Cro-SEM), respectively. The results indicated the HIPEs stabilized by OSAS/EGCG mixtures exhibited good environmental stability. All HIPEs displayed excellent antioxidant properties. As the concentration of EGCG in the OSAS/EGCG mixtures was increased from 0 to 0.2 % (w/v), the lipid hydroperoxide (LH) content was reduced from 534.89 mmol/kg oil to 453.77 mmol/kg oil, and malondialdehyde (MDA) content was reduced from 10.08 mmol/kg oil to 6.07 mmol/kg oil. Combined with the effect of NaCl, the oxidative stability was improved further. This study provided a new formulation of food-grade O/W HIPEs with strong antioxidant properties could be a potential solid fat substitute for future foods.

Keywords: (−)-Epigallocatechin-3-gallate; Environmental stability; High internal phase emulsions; Octenyl succinic anhydride modified starch; Oxidative stability.