DNAzyme assisted single amplification for FEN1 activity detection using a personal glucose meter

Mingxi Li; Weijie Zang; Shuiliang Wang; Zhannuo Lin; Shu Liu; Yiling Chen; Xiaohui Ruan; Yuanfang Luo; Chao Xing; Chunhua Lu

doi:10.1016/j.aca.2024.343495

DNAzyme assisted single amplification for FEN1 activity detection using a personal glucose meter

Anal Chim Acta. 2025 Jan 22:1336:343495. doi: 10.1016/j.aca.2024.343495. Epub 2024 Nov 28.

Authors

Mingxi Li¹, Weijie Zang², Shuiliang Wang³, Zhannuo Lin⁴, Shu Liu⁴, Yiling Chen⁴, Xiaohui Ruan⁴, Yuanfang Luo⁴, Chao Xing⁵, Chunhua Lu⁶

Affiliations

¹ MOE Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, 350116, PR China; Fujian Key Laboratory of Functional Marine Sensing Materials, Center for Advanced Marine Materials and Smart Sensors, College of Materials and Chemical Engineering, Minjiang University, Fuzhou, 350108, PR China.
² MOE Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, 350116, PR China.
³ Fujian Key Laboratory of Aptamers Technology, Affiliated Dongfang Hospital (the 900th Hospital) of School of Medicine, Xiamen University, Fuzhou, 350025, PR China.
⁴ Fujian Key Laboratory of Functional Marine Sensing Materials, Center for Advanced Marine Materials and Smart Sensors, College of Materials and Chemical Engineering, Minjiang University, Fuzhou, 350108, PR China.
⁵ MOE Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, 350116, PR China; Fujian Key Laboratory of Functional Marine Sensing Materials, Center for Advanced Marine Materials and Smart Sensors, College of Materials and Chemical Engineering, Minjiang University, Fuzhou, 350108, PR China. Electronic address: [email protected].
⁶ MOE Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, 350116, PR China. Electronic address: [email protected].

PMID: 39788665
DOI: 10.1016/j.aca.2024.343495

Abstract

Flap endonuclease 1 (FEN1) plays a vital role in cancer by modulating DNA repair mechanisms, inducing genomic instability, and serving as a promising biomarker for cancer diagnosis and prognosis. In this work, we present the development of a novel DNAzyme signal amplification-directed point-of-care sensing system (Dz-PGM) for the sensitive and specific detection of FEN1. The Dz-PGM system utilizes DNAzyme signal amplification in conjunction with a personal glucose meter (PGM) for reporting, capitalizing on a biochemical cascade initiated by FEN1 recognition. Benefiting from enzymatic recognition, magnetic separation, and DNAzyme based signal amplification, this Dz-PGM system demonstrates exceptional specificity and achieves a low detection limit of 3.56× 10^-2 U/mL for FEN1. The versatility of the system is further demonstrated through its ability to screen FEN1 inhibitor drugs and to distinguish FEN1 levels in real cell samples with high accuracy. The article underscores the potential of the Dz-PGM system as a promising tool for early cancer diagnosis, and in constructing sensitive detection platforms for DNA repair enzymes.

Keywords: DNA repair enzymes; DNAzyme; FEN1; Personal glucose meter; Signal amplification.

MeSH terms

Biosensing Techniques / methods
Blood Glucose Self-Monitoring
DNA, Catalytic* / chemistry
DNA, Catalytic* / metabolism
Flap Endonucleases* / metabolism
Humans
Limit of Detection
Nucleic Acid Amplification Techniques

Substances

Flap Endonucleases
DNA, Catalytic
FEN1 protein, human