Presence of polyketide synthases and nonribosomal peptide synthetase in culturable bacteria associated with Aplysina fulva and Aplysina caissara (Porifera)

Letícia Sanfilippo Rojas; Michelle Guzmán de Fernandes; Andressa Cristina Ramaglia da Motta; Luiza Porteiro Carminato; Ana Caroline Zanatta; Gisele Lôbo-Hajdu; Paula Jimenez; Márcio Reis Custódio; Cristiane Cassiolato Pires Hardoim

doi:10.1007/s42770-024-01588-4

Presence of polyketide synthases and nonribosomal peptide synthetase in culturable bacteria associated with Aplysina fulva and Aplysina caissara (Porifera)

Braz J Microbiol. 2025 Jan 10. doi: 10.1007/s42770-024-01588-4. Online ahead of print.

Authors

Letícia Sanfilippo Rojas¹, Michelle Guzmán de Fernandes², Andressa Cristina Ramaglia da Motta³, Luiza Porteiro Carminato³, Ana Caroline Zanatta⁴, Gisele Lôbo-Hajdu⁵, Paula Jimenez⁶, Márcio Reis Custódio², Cristiane Cassiolato Pires Hardoim^{7

8}

Affiliations

¹ Graduate Program in Evolution and Diversity, Federal University of ABC, Av. dos Estados, Bairro Bangu, Santo André, São Paulo, 5001, CEP 09210-580, Brazil.
² Department of Physiology, Institute of Biosciences, University of São Paulo, Rua do Matão, Travessa 14, 101, São Paulo, CEP 05508-090, Brazil.
³ São Paulo State University, Praça Infante Dom Henrique s/nº, Parque Bitaru, São Vicente, São Paulo, CEP 11.330-900, Brazil.
⁴ Institute of Chemistry, São Paulo State University, Rua Prof. Francisco Degni, 55, Araraquara, São Paulo, CEP 14800-060, Quitandinha, Brazil.
⁵ Department of Genetics, Biology Institute Roberto Alcântara Gomes, Rio de Janeiro State University, Rua São Francisco Xavier, 524, Maracanã, Rio de Janeiro, CEP: 20550-013, Brazil.
⁶ Institute of Marine Sciences, Federal University of São Paulo, Campus Baixada Santista, Rua Dr. Carvalho de Mendonça, 144, Vila Belmiro, Santos, São Paulo, 11070-100, Brazil.
⁷ Graduate Program in Evolution and Diversity, Federal University of ABC, Av. dos Estados, Bairro Bangu, Santo André, São Paulo, 5001, CEP 09210-580, Brazil. [email protected].
⁸ Department of Physiology, Institute of Biosciences, University of São Paulo, Rua do Matão, Travessa 14, 101, São Paulo, CEP 05508-090, Brazil. [email protected].

PMID: 39792328
DOI: 10.1007/s42770-024-01588-4

Abstract

Culture-dependent and -independent studies have provided access to symbiont genes and the functions they play for host sponges. Thus, this work investigates the diversity, presence of genes of pharmacological interest, biological activities and metabolome of the bacteria isolated from the sponges Aplysina caissara and Aplysina fulva collected on the southwestern Atlantic Coast. The genes for Polyketide Synthases types I and II and Nonribosomal Peptide Synthetases were screened in more than 200 bacterial strains obtained, from which around 40% were putatively novel. Twenty-two were positive for at least one of the genes screened. Among them, 12 exhibited antimicrobial activities and one inhibited the proliferation of cancer cells. The metabolic profiles of the 22 strains were analyzed by liquid chromatography with tandem mass spectrometry and molecular network. The Global Natural Products Social Molecular Networking MolNetEnhancer workflow provided a more comprehensive understanding of the metabolic profiles. The results revealed the existence of a wide range of metabolites, however more than half of the compounds could not be identified. It was further observed that the metabolic diversity among the strains varied primarily due to the cultivation medium used. Together the results obtained here revealed the pharmacological potential of the bacteria isolated from Aplysina species.

Keywords: NRPS; PKS; Bioactive compounds; GNPS; Molecular network; Sponges.

Abstract

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