Chromosome-level genome assembly of Megachile sculpturalis Smith (Hymenoptera, Apoidea, Megachilidae)

Guang Yang; Qingsong Zhou; Juanjuan Yang; Guoai Chen; Zeqing Niu; Michael Orr; Rafael Rodrigues Ferrari; Yihan Zhang; Xiaoyu Shi; Rui Cheng; Chaodong Zhu; Arong Luo

doi:10.1038/s41597-025-04385-2

Chromosome-level genome assembly of Megachile sculpturalis Smith (Hymenoptera, Apoidea, Megachilidae)

Sci Data. 2025 Jan 10;12(1):46. doi: 10.1038/s41597-025-04385-2.

Authors

Guang Yang^#^{1

2}, Qingsong Zhou^#¹, Juanjuan Yang¹, Guoai Chen^{1

2}, Zeqing Niu¹, Michael Orr^{1

3}, Rafael Rodrigues Ferrari^{1

4}, Yihan Zhang^{1

2}, Xiaoyu Shi¹, Rui Cheng¹, Chaodong Zhu^{5

6

7

8}, Arong Luo^{9

10}

Affiliations

¹ Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
² College of Biological Sciences, University of Chinese Academy of Science, Beijing, China.
³ Entomologie, Staatliches Museum für Naturkunde Stuttgart, Stuttgart, Germany.
⁴ Centro de Formação em Ciências Ambientais, Universidade Federal do Sul da Bahia, Porto Seguro, Brazil.
⁵ Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing, China. [email protected].
⁶ College of Biological Sciences, University of Chinese Academy of Science, Beijing, China. [email protected].
⁷ International College, University of Chinese Academy of Sciences, Beijing, China. [email protected].
⁸ State Key Laboratory of Integrated Pest Management, Institute of Zoology, Chinese Academy of Sciences, Beijing, China. [email protected].
⁹ Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing, China. [email protected].
¹⁰ International College, University of Chinese Academy of Sciences, Beijing, China. [email protected].

^# Contributed equally.

Abstract

Megachile sculpturalis Smith, 1853 native to East Asia, is an important solitary bee species that has invaded both Europe and the United States. This study provides the first chromosome-level genome assembly of M. sculpturalis using a combination of Nanopore long reads, Illumina short reads, and Hi-C data. The genome comprises 296.99 Mb distributed across 16 chromosomes. N50, L50 and BUSCO completeness reached 19.128 Mb, 7 scaffolds, and 96.7%, respectively. The genome contains 104 Mb repetitive elements (35.02% of the assembly size) and 11,446 predicted protein-coding genes. This chromosome-level genome will serve as an essential genomic resource for future research on Megachilidae.

Publication types

Dataset

MeSH terms

Animals
Bees / genetics
Chromosomes, Insect / genetics
Genome, Insect*

Grants and funding

32070465/National Natural Science Foundation of China (National Science Foundation of China)