Discovery of potentially degrading microflora of different types of plastics based on long-term in-situ incubation in the deep sea

Plastic waste that ends up in the deep sea is becoming an increasing concern. However, it remains unclear whether there is any microflora capable of degrading plastic within this vast ecosystem. In this study, we investigated the bacterial communities associated with different types of plastic-polyamide-nylon 4, 6 (PA), polyethylene (PE), polyethylene terephthalate (PET), and polystyrene (PS)-after one year of in situ incubation in the pelagic deep sea of the Western Pacific. The study was conducted via a submarine mooring system, anchored at four sites with water depths ranging from 1,167 to 1,735 meters in an area of seamounts. High-throughput 16S rRNA gene sequencing revealed distinct bacterial diversities associated with specific plastic types and locations. The family Gordoniaceae was enriched by PS and PE plastics, while the abundance of Methyloligellaceae was significantly increased in the presence of PET. In the case of PA, Bdellovibrionaceae was enriched. Additionally, all plastic types promoted the relative abundance of Rhodobacteraceae and Sulfurimonadaceae families. Plastics appeared to stimulate bacterial communities involved in nitrate and sulfur cycling in seawater, suggesting that nitrogen and sulfur potentially play significant roles in plastic degradation in deep-sea environments. The dominant family Kordiimonadaceae was identified as a significantly different taxon in non-plastic seawater. Furthermore, the addition of plastics enhanced negative interactions among the bacterial communities in the surrounding seawater, with Proteobacteria and Bdellovibrionota selected for the core microbiome. Overall, this in situ deep-sea incubation revealed the response of indigenous microflora to man-made polymeric materials and highlighted the bacterial communities that may be involved in plastic degradation in oceanic areas.