Live-cell metabolic analyzer protocol for measuring glucose and lactate metabolic changes in human cells

Kenji Miki; Mikako Yagi; Ko Igami; Hiroki Kittaka; Akito Tani; Natsuki Horiuchi; Satoshi Fukumoto; Koji Yoshimoto; Takeshi Uchiumi

doi:10.1016/j.xpro.2024.103518

Live-cell metabolic analyzer protocol for measuring glucose and lactate metabolic changes in human cells

STAR Protoc. 2025 Jan 10;6(1):103518. doi: 10.1016/j.xpro.2024.103518. Online ahead of print.

Authors

Kenji Miki¹, Mikako Yagi², Ko Igami³, Hiroki Kittaka³, Akito Tani², Natsuki Horiuchi², Satoshi Fukumoto⁴, Koji Yoshimoto⁵, Takeshi Uchiumi⁶

Affiliations

¹ Department of Health Sciences, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka 812-8582, Japan; Department of Neurosurgery, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka 812-8582, Japan.
² Department of Health Sciences, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka 812-8582, Japan.
³ Kyushu Pro Search Limited Liability Partnership, Fukuoka, Japan.
⁴ PHC Corporation, Ehime, Japan.
⁵ Department of Neurosurgery, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka 812-8582, Japan.
⁶ Department of Health Sciences, Graduate School of Medical Sciences, Kyushu University, Higashi-ku, Fukuoka 812-8582, Japan. Electronic address: [email protected].

PMID: 39799573
DOI: 10.1016/j.xpro.2024.103518

Abstract

Understanding metabolic conditions related to glycolysis dependence is crucial for developing new treatments in cancer and regenerative medicine. This protocol details a method for using the live-cell metabolic analyzer (LiCellMo) to measure continuous changes in glucose consumption and lactate production in cultured human cells. LiCellMo provides real-time data on consecutive metabolic changes, improving measurements of these processes in various contexts, including in cancer and regenerative treatments.

Keywords: cell biology; cell culture; metabolism.