Determination of five alternaria toxins in peppermint by dispersive solid-phase extraction coupled with ultra-high performance liquid chromatography-tandem mass spectrometry based on MOF-808-TFA

Minhai Liu; Yan Zhang; Rui Cheng; Ting Tan; Lan Guo; Fan Liu; Yiqun Wan

doi:10.1016/j.foodchem.2025.142822

Determination of five alternaria toxins in peppermint by dispersive solid-phase extraction coupled with ultra-high performance liquid chromatography-tandem mass spectrometry based on MOF-808-TFA

Food Chem. 2025 Jan 8:471:142822. doi: 10.1016/j.foodchem.2025.142822. Online ahead of print.

Authors

Minhai Liu¹, Yan Zhang², Rui Cheng¹, Ting Tan³, Lan Guo³, Fan Liu⁴, Yiqun Wan⁵

Affiliations

¹ School of Chemistry and Chemical Engineering, Nanchang University, Nanchang 330031, People's Republic of China.
² School of Pharmacy, Jiangxi University of Chinese Medicine, Nanchang 330004, People's Republic of China.
³ Center of Analysis and Testing, Nanchang University, Nanchang 330031, People's Republic of China.
⁴ Center of Analysis and Testing, Nanchang University, Nanchang 330031, People's Republic of China; State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330031, People's Republic of China.
⁵ School of Chemistry and Chemical Engineering, Nanchang University, Nanchang 330031, People's Republic of China; State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330031, People's Republic of China. Electronic address: [email protected].

PMID: 39799690
DOI: 10.1016/j.foodchem.2025.142822

Abstract

An efficient and rapid ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MSMS) method was developed for simultaneous determination of 5 alternaria toxins (ATs) in edible and medicinal plant - peppermint using MOF-808-trifluoroacetic acid (MOF-808-TFA) as the adsorbent. Characterization methods such as scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) and N₂ adsorption-desorption demonstrated that the synthesized MOF-808-TFA had a regular ortho-octahedral configuration and high specific surface area. Under the optimal conditions, the 5 ATs showed good linearity (R² ≥ 0.9993) in their respective concentration ranges. The limits of quantifications (LOQs) of the method ranged from 0.21 to 0.48 μg/L, and the recoveries were 77.8-118.2 %, with the relative standard deviations (RSDs) less than 8.9 %.

Keywords: D-SPE; MOF-808-TFA; Peppermint; alternaria.