Organ-on-a-chip (OoC) is a breakthrough technology in biomedicine. As microphysiological systems constructed in vitro, OoCs can simulate the main structures and functions of human organs, thereby providing a powerful tool for drug screening and disease model construction. Furthermore, the coupling of OoCs and sensors has been an innovative discovery in the field of biomedical and electronic engineering in recent years. The integration of sensors into OoCs allows the real-time monitoring of the changes in the microenvironmental parameters within the chip, reflecting the physiological responses of cells or tissues in the OoC and providing more accurate data support for drug development and disease treatment. In this work, we briefly outline the design ideas of OoCs, summarize the commonly used materials for OoCs and their advantages and disadvantages, and provide the most recent practical examples of the combination of OoCs and sensors in pharmaceutical and medical sciences. Furthermore, perspectives, challenges and their solutions in the future development of this technology are provided, with the aim to inspire the researchers to work toward the subsequent development of OoCs having improved reliability.