Enzyme cascade nanozyme based colorimetric sensor for detection of uric acid as a biomarker of hyperuricemia

Wenrui Ke; Guochen Kuang; Chuangqi Gu; Junhao Wang; Xiao Jiang; Rongjiao Zhu; Haiyuan Wang; Zhen Zhang

doi:10.1007/s00604-024-06946-5

Enzyme cascade nanozyme based colorimetric sensor for detection of uric acid as a biomarker of hyperuricemia

Mikrochim Acta. 2025 Jan 15;192(2):85. doi: 10.1007/s00604-024-06946-5.

Authors

Wenrui Ke^{1

2}, Guochen Kuang^{1

2}, Chuangqi Gu^{1

2}, Junhao Wang^{1

2}, Xiao Jiang^{1

2}, Rongjiao Zhu^{3

4}, Haiyuan Wang^{5

6}, Zhen Zhang^{7

8}

Affiliations

¹ Key Laboratory of Organic Integrated Circuit, Ministry of Education & Tianjin Key Laboratory of Molecular Optoelectronic Sciences, Department of Chemistry, School of Science, Tianjin University, Tianjin, 300072, China.
² Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, 300072, China.
³ Key Laboratory of Organic Integrated Circuit, Ministry of Education & Tianjin Key Laboratory of Molecular Optoelectronic Sciences, Department of Chemistry, School of Science, Tianjin University, Tianjin, 300072, China. [email protected].
⁴ Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, 300072, China. [email protected].
⁵ Key Laboratory of Organic Integrated Circuit, Ministry of Education & Tianjin Key Laboratory of Molecular Optoelectronic Sciences, Department of Chemistry, School of Science, Tianjin University, Tianjin, 300072, China. [email protected].
⁶ Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, 300072, China. [email protected].
⁷ Key Laboratory of Organic Integrated Circuit, Ministry of Education & Tianjin Key Laboratory of Molecular Optoelectronic Sciences, Department of Chemistry, School of Science, Tianjin University, Tianjin, 300072, China. [email protected].
⁸ Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin, 300072, China. [email protected].

PMID: 39812877
DOI: 10.1007/s00604-024-06946-5

Abstract

A Cr-doped VO₂ nanobelt (Cr/VO₂) with remarkable peroxidase-like activity was synthesized and coupled with uricase to catalyze the cascade reaction for detection of uric acid. Notably, the affinity of Cr/VO₂ for 3,3',5,5'-tetramethylbenzidine dihydrochloride hydrate (TMB) and hydrogen peroxide (H₂O₂) is tenfold and 20-fold higher, respectively, than that of horseradish peroxidase (HRP). The Cr/VO₂ exhibits highly reactive and stable peroxidase activity at temperatures of 20-60 ℃. Thus, the reaction catalyzed by Cr/VO₂ can be carried out at room temperature, greatly simplifying the testing process. This enzyme cascade nanozyme based colorimetric assay enables the selective and simple detection of uric acid within 30 min with a detection limit of only 0.34 μM, and has an observable potential in clinical applications.

Keywords: Cascade reaction; Colorimetric detection; Nanozyme; Uric acid.

MeSH terms

Benzidines* / chemistry
Biomarkers* / analysis
Biomarkers* / blood
Biosensing Techniques / methods
Chromogenic Compounds / chemistry
Colorimetry* / methods
Humans
Hydrogen Peroxide* / chemistry
Hyperuricemia* / blood
Hyperuricemia* / diagnosis
Limit of Detection*
Nanostructures / chemistry
Urate Oxidase* / chemistry
Uric Acid* / blood
Uric Acid* / chemistry

Substances

Uric Acid
Urate Oxidase
Hydrogen Peroxide
Benzidines
3,3',5,5'-tetramethylbenzidine
Biomarkers
Chromogenic Compounds

Abstract

MeSH terms

Substances

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