Dual-Selection Strategy for Generating Knock-Out Lines of Human Embryonic Stem Cells

J Cell Mol Med. 2025 Jan;29(2):e70259. doi: 10.1111/jcmm.70259.

Ziyu Zhou^#¹, Lingling Tong^#¹, Yunbing Chen^#¹, Ruoming Wang¹, Yu Shen¹, Di Chen^{1

2

3}

¹ Center for Reproductive Medicine of the Second Affiliated Hospital, Center for Regeneration and Cell Therapy of Zhejiang University-University of Edinburgh Institute (ZJU-UoE Institute), Zhejiang University School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
² State Key Laboratory of Biobased Transportation Fuel Technology, Haining, Zhejiang, China.
³ Dr. Li Dak sum & yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Hangzhou, Zhejiang, China.

^# Contributed equally.

No abstract available

Keywords: CRISPR/Cas9; Knock‐out; dual selection; human embryonic stem cells.

Grants and funding