Antigenicity evaluation of the synthetic trisaccharides related to Plesiomonas shigelloides serotype O51 O-antigen containing an acetamidino group

Chunjun Qin; Wei Han; Guangzong Tian; Xiaopeng Zou; Anna Maciejewska; Jolanta Lukasiewicz; Peter H Seeberger; Jing Hu; Jian Yin

doi:10.1016/j.carres.2025.109388

Antigenicity evaluation of the synthetic trisaccharides related to Plesiomonas shigelloides serotype O51 O-antigen containing an acetamidino group

Carbohydr Res. 2025 Jan 16:550:109388. doi: 10.1016/j.carres.2025.109388. Online ahead of print.

Authors

Chunjun Qin¹, Wei Han², Guangzong Tian¹, Xiaopeng Zou¹, Anna Maciejewska³, Jolanta Lukasiewicz³, Peter H Seeberger⁴, Jing Hu⁵, Jian Yin⁶

Affiliations

¹ School of Biotechnology and Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, Jiangnan University, Lihu Ave. 1800, Wuxi, 214122, China; Biomolecular Systems Department, Max Planck Institute of Colloids and Interfaces, Am Mühlenberg 1, Potsdam, 14476, Germany; Innovation Center for Vaccine Engineering, Jiangnan University, Lihu Ave. 1800, Wuxi, 214122, China.
² School of Biotechnology and Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, Jiangnan University, Lihu Ave. 1800, Wuxi, 214122, China; Innovation Center for Vaccine Engineering, Jiangnan University, Lihu Ave. 1800, Wuxi, 214122, China.
³ Laboratory of Microbial Immunochemistry and Vaccines, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wroclaw, Poland.
⁴ Biomolecular Systems Department, Max Planck Institute of Colloids and Interfaces, Am Mühlenberg 1, Potsdam, 14476, Germany.
⁵ Wuxi School of Medicine, Jiangnan University, Lihu Ave. 1800, Wuxi, 214122, China; Innovation Center for Vaccine Engineering, Jiangnan University, Lihu Ave. 1800, Wuxi, 214122, China. Electronic address: [email protected].
⁶ School of Biotechnology and Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, Jiangnan University, Lihu Ave. 1800, Wuxi, 214122, China; School of Life Sciences and Health Engineering, Jiangnan University, Lihu Ave. 1800, Wuxi, 214122, China; Innovation Center for Vaccine Engineering, Jiangnan University, Lihu Ave. 1800, Wuxi, 214122, China. Electronic address: [email protected].

PMID: 39832422
DOI: 10.1016/j.carres.2025.109388

Abstract

Bacterial surface polysaccharides are widely decorated with diverse rare functional groups. An understanding of the role of these functional groups in immunological activity of the glycans is required for the development of carbohydrate-based treatment against bacterial infectious diseases. Here, the antigenicity evaluation of synthetic trisaccharide 1 related to Plesiomonas shigelloides serotype O51 lipopolysaccharide (LPS) O-antigen and its acetamido derivative 2 was achieved to elucidate the immunological importance of O-antigen structure. The rabbit antisera with high titers of IgG and IgM were collected after immunization with purified P. shigelloides serotype O51 LPS. Glycan microarray screening results showed that both IgG and IgM in rabbit antisera bound specifically to trisaccharides 1 and 2, indicating that the trisaccharide repeating unit is a key antigenic epitope of P. shigelloides serotype O51 O-antigen, while the acetamidino group has no influence on the antigenicity. This finding provides key information for glycoconjugate vaccine design for P. shigelloides serotype O51.

Keywords: Acetamidino group; Antigenicity; Glycan microarray; O-Antigen; Plesiomonas shigelloides; Synthetic oligosaccharide.