Therapeutic Potential of Shilong Qingxue Granule and Its Extract Against Glutamate Induced Neural Injury: Insights from In Vivo and In Vitro Models

Nan Hu; Cheng Zeng; Yi Cao; Xuehao Li; Fei Bai; Jinhui Wang; Baofeng Yang; Chunli Li

doi:10.1016/j.jep.2025.119396

Therapeutic Potential of Shilong Qingxue Granule and Its Extract Against Glutamate Induced Neural Injury: Insights from In Vivo and In Vitro Models

J Ethnopharmacol. 2025 Jan 21:119396. doi: 10.1016/j.jep.2025.119396. Online ahead of print.

Authors

Nan Hu¹, Cheng Zeng², Yi Cao², Xuehao Li², Fei Bai³, Jinhui Wang⁴, Baofeng Yang⁵, Chunli Li⁶

Affiliations

¹ Department of Pharmacology, Shenyang Pharmaceutical University, Shenyang, Liaoning, China; College of Pharmacy, Shenzhen Technology University, Shenzhen, Guangdong, China.
² Department of Pharmacology, Shenyang Pharmaceutical University, Shenyang, Liaoning, China.
³ College of Pharmacy, Shenzhen Technology University, Shenzhen, Guangdong, China; Department of Pharmacy, Harbin Medical University, Harbin, 150081, Heilongjiang, China.
⁴ Department of Pharmacy, Harbin Medical University, Harbin, 150081, Heilongjiang, China.
⁵ Department of Pharmacology, Shenyang Pharmaceutical University, Shenyang, Liaoning, China; Department of Pharmacy, Harbin Medical University, Harbin, 150081, Heilongjiang, China.
⁶ Department of Pharmacology, Shenyang Pharmaceutical University, Shenyang, Liaoning, China; College of Pharmacy, Shenzhen Technology University, Shenzhen, Guangdong, China. Electronic address: [email protected].

PMID: 39848417
DOI: 10.1016/j.jep.2025.119396

Abstract

Ethnopharmacological relevance: Shilong Qingxue Granule (SQG), a traditional Chinese medicine, effectively treats the secondary neurological damage and functional deficits caused by cerebral hemorrhage, though its exact mechanism remains unclear.

Aim of the study: This study aimed to investigate the effects of SQG and its mechanisms.

Materials and methods: we evaluated the effects of SQG and its extracts on glutamate induced nerve damage using in vivo and in vitro models. Brain water content was measured and brain tissue was stained with hematoxylin and eosin (HE) to evaluate the brain protective effect of SQG in rats. HPLC and UPLC-Q-TOF-MS were used to identify the chemical components in SQG. The model of PC12 cells induced by glutamate was established to detect intracellular Ca²⁺ and mitochondrial membrane potential (MMP), the content of intracellular reactive oxygen species (ROS), acridine orange/ethidium bromide (AO/EB), and the possible mechanism of action in vivo was explored by western blot and RNA sequencing.

Results: SQG alleviates brain edema and neuronal damage in glutamate induced rats by modulating mitochondrial apoptotic and MAPK signaling pathways. The SQG extract was separated by silica gel chromatographic column to obtain 20 components, and the S-18 improves PC12 survival under glutamate induced conditions by MMP, reducing ROS and Ca²⁺ levels, and protecting against cell body and nucleus damage to against apoptosis.

Conclusion: SQG and its extract demonstrate protective effects against glutamate induced nerve injury in vivo and in vitro, suggesting potential therapeutic benefits for neurological disorders involving glutamate excitotoxicity.

Keywords: Apoptosis; Glutamate; MAPKs; Shilong Qingxue Granule.