Protocol for real-time assessment of energy metabolism in dissociated mouse retinal photoreceptors using a SeahorseXFe24 analyzer

Maria Yera; Joshua J Wang; Sarah X Zhang

doi:10.1016/j.xpro.2025.103595

Protocol for real-time assessment of energy metabolism in dissociated mouse retinal photoreceptors using a SeahorseXFe24 analyzer

STAR Protoc. 2025 Jan 23;6(1):103595. doi: 10.1016/j.xpro.2025.103595. Online ahead of print.

Authors

Maria Yera¹, Joshua J Wang², Sarah X Zhang³

Affiliations

¹ Department of Ophthalmology and Ross Eye Institute, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, State University of New York, Buffalo, NY, USA. Electronic address: [email protected].
² Department of Ophthalmology and Ross Eye Institute, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, State University of New York, Buffalo, NY, USA.
³ Department of Ophthalmology and Ross Eye Institute, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, State University of New York, Buffalo, NY, USA. Electronic address: [email protected].

PMID: 39854204
DOI: 10.1016/j.xpro.2025.103595

Abstract

Defects in retinal metabolism have been linked to the onset and progression of various retinal diseases. Herein, we provide a protocol for measuring bioenergetics in dissociated mouse retinal photoreceptors. We outline detailed instructions for obtaining morphologically intact and viable photoreceptor cells from adult mice and preparing the cells for metabolic analysis using a SeahorseXFe24 analyzer. This protocol allows a real-time assessment of mitochondrial respiration and glycolysis in retinal photoreceptors in response to genetic modifications or pathological insults in mouse models.

Keywords: Cell Biology; Metabolism; Neuroscience.