Unveiling the role of OsSAP17: Enhancing plant resistance to drought and salt

Wenjun Jiang; Yang Shi; Zhiye Du; Yingxu Zhou; Longying Wu; Ji Chen; Yanyan Huang; Lijuan Wu; Yueyang Liang; Zhonghui Zhang; Vinod Kumar; Zhi Chen; Deqiang Li; Jin Huang

doi:10.1016/j.plaphy.2024.109451

Unveiling the role of OsSAP17: Enhancing plant resistance to drought and salt

Plant Physiol Biochem. 2024 Dec 28:220:109451. doi: 10.1016/j.plaphy.2024.109451. Online ahead of print.

Authors

Wenjun Jiang¹, Yang Shi², Zhiye Du³, Yingxu Zhou¹, Longying Wu¹, Ji Chen⁴, Yanyan Huang⁵, Lijuan Wu⁴, Yueyang Liang⁵, Zhonghui Zhang⁶, Vinod Kumar⁷, Zhi Chen¹, Deqiang Li⁵, Jin Huang⁸

Affiliations

¹ College of Ecology and Environment, Chengdu University of Technology, Sichuan, 610059, China.
² College of Ecology and Environment, Chengdu University of Technology, Sichuan, 610059, China; College of Resources, Sichuan Agricultural University, Sichuan, 611130, China.
³ School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China.
⁴ College of Agronomy, Sichuan Agricultural University, Sichuan, 611130, China.
⁵ State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Sichuan, 611130, China.
⁶ Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Science, South China Normal University, Guangzhou, 510631, China.
⁷ Algal Research and Bioenergy Laboratory, Department of Food Science and Technology, Graphic Era (Deemed to be University), Dehradun, Uttarakhand, 248002, India.
⁸ College of Ecology and Environment, Chengdu University of Technology, Sichuan, 610059, China; Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, Lanzhou University, Lanzhou, 730000, China; Key Laboratory of Monitoring for Heavy Metal Pollutants, Ministry of Ecology and Environment, Hunan, 410019, China. Electronic address: [email protected].

PMID: 39854789
DOI: 10.1016/j.plaphy.2024.109451

Abstract

With the intensification of climate change coupled with the inadequate agricultural management in certain regions, plants face numerous challenges due to various abiotic stresses. Stress associated proteins (SAPs) are essential functional genes in plants for coping with stress. This research provides a functional analysis of OsSAP17, a protein belonging to the SAP family in rice. The expression level of OsSAP17 was induced under drought, salt stress and ABA treatment. Subcellular localization analysis revealed that the OsSAP17 protein was distributed in both the cytoplasm and nucleus. The ectopic expression of OsSAP17 significantly increased the capacity to withstand drought and salt stress in both transgenic yeast and Arabidopsis. Additionally, the ectopic expression of OsSAP17 led to notable changes in the expression of Arabidopsis ABA-related genes, including AtNCED3, AtABA2, and AtSnRK2.2. These results indicated that OsSAP17 was able to positively regulate drought and salt tolerance in plants. The insights from this study provided a fundamental understanding of the role of OsSAP17 in abiotic stress response mechanisms and were potentially valuable for breeding crops with enhanced stress tolerance.

Keywords: ABA signaling; Drought stress; Heterologous expression; OsSAP17; Salt stress.