3-Methoxy-4-aminoazobenzene, a selective inducer for a high spin form of cytochrome P-448 in rat liver microsomes

Biochem Biophys Res Commun. 1985 Dec 31;133(3):1072-7. doi: 10.1016/0006-291x(85)91245-8.

Abstract

Treatment of Sprague Dawley rats with 3-methoxy-4-aminoazobenzene (3-MeO-AAB) resulted in striking increase of the activity of hepatic microsomal cytochrome P-450s which could efficiently catalyze the mutagenic activation of hepatocarcinogenic aromatic amines such as a tryptophan-pyrolysate component, Trp P-2, and a glutamic acid-pyrolysate component, Glu P-1. The 3-MeO-AAB-induced cytochrome P-450 (3-MeO-AAB-P-450) was examined for the molecular character by immuno-Western blotting using monoclonal antibody to 3-methylcholanthrene-induced cytochrome P-448 (P-448H; m.w. 54,000).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Azo Compounds / pharmacology*
  • Catalysis
  • Cytochrome P-450 CYP1A2
  • Cytochrome P-450 Enzyme System / biosynthesis
  • Cytochromes / biosynthesis*
  • Electrophoresis, Polyacrylamide Gel
  • Enzyme Induction / drug effects
  • Immunochemistry
  • Male
  • Microsomes, Liver / enzymology*
  • Mutagenicity Tests
  • Rats
  • Rats, Inbred Strains
  • p-Aminoazobenzene / analogs & derivatives
  • p-Aminoazobenzene / pharmacology*

Substances

  • Azo Compounds
  • Cytochromes
  • p-Aminoazobenzene
  • 3-methoxy-4-aminoazobenzene
  • Cytochrome P-450 Enzyme System
  • Cyp1a2 protein, rat
  • Cytochrome P-450 CYP1A2