Abstract
Reduced Fab' fragments of viral antibody hybridized with reduced Fab' fragments of antiferritin immunoglobulin G bind to viral antigenic sites in the plasma membrane of L cells infected with vesicular stomatitis virus. The hybrid antibody reacts specifically with ferritin, which can be identified by electron microscopy, and with fluorescein-conjugated apoferritin, which can be identified by fluorescence microscopy.
MeSH terms
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Animals
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Antibodies*
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Antigens / isolation & purification*
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Cell Membrane / immunology*
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Ferritins
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Fluorescent Antibody Technique*
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Immune Sera
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Immunochemistry
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Immunoglobulin G
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L Cells / immunology*
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Methods
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Microscopy, Electron
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Microscopy, Fluorescence
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Rabbits
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Staining and Labeling
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Time Factors
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Vesicular stomatitis Indiana virus / immunology*
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Vesicular stomatitis Indiana virus / pathogenicity
Substances
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Antibodies
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Antigens
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Immune Sera
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Immunoglobulin G
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Ferritins