Adenylate-rich sequences in Sendai virus transcripts from infected cells

J Virol. 1972 Aug;10(2):314-7. doi: 10.1128/JVI.10.2.314-317.1972.

Abstract

Virus-specific complementary ribonucleic acid (RNA) from cells infected with Sendai virus was isolated by a procedure involving hybridization with virion RNA and isopycnic centrifugation of the RNA hybrids. The complementary RNA contained adenylate-rich sequences which sedimented at about 4S.

MeSH terms

  • Adenine / metabolism
  • Adenosine Monophosphate / analysis*
  • Animals
  • Base Sequence
  • Centrifugation, Density Gradient
  • Chemical Precipitation
  • Chick Embryo
  • Culture Techniques
  • Dactinomycin
  • Genetics, Microbial
  • Lung
  • Nucleic Acid Hybridization
  • Parainfluenza Virus 1, Human / analysis*
  • Parainfluenza Virus 1, Human / growth & development
  • Parainfluenza Virus 1, Human / metabolism
  • Potassium Iodide
  • RNA, Viral / analysis*
  • RNA, Viral / biosynthesis
  • RNA, Viral / isolation & purification
  • Ribonucleases
  • Sodium Dodecyl Sulfate
  • Sucrose
  • Transcription, Genetic*
  • Tritium
  • Uridine / metabolism
  • Virus Replication

Substances

  • RNA, Viral
  • Tritium
  • Potassium Iodide
  • Dactinomycin
  • Sodium Dodecyl Sulfate
  • Adenosine Monophosphate
  • Sucrose
  • Ribonucleases
  • Adenine
  • Uridine