A new method using 'proteinase K' to prevent mRNA degradation during isolation from HeLa cells

Biochem Biophys Res Commun. 1971 Jul 16;44(2):513-9. doi: 10.1016/0006-291x(71)90632-2.
No abstract available

MeSH terms

  • Carbonates
  • Centrifugation, Density Gradient
  • Chemical Phenomena
  • Chemistry
  • Ethanol
  • HeLa Cells / analysis*
  • Hydrolysis
  • Kinetics
  • Methods
  • Peptide Hydrolases
  • RNA
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / isolation & purification*
  • RNA, Ribosomal / isolation & purification
  • Ribonucleases / antagonists & inhibitors
  • Temperature
  • Tritium
  • Uridine / metabolism
  • Yeasts / enzymology

Substances

  • Carbonates
  • RNA, Messenger
  • RNA, Ribosomal
  • Tritium
  • Ethanol
  • RNA
  • Ribonucleases
  • Peptide Hydrolases
  • Uridine