Abstract
A new procedure has been worked out to establish restriction maps. The method is fast, does not in general require labeled DNA and has been applied to map the linear palindromic rDNA of Physarum with the restriction enzyme Bst EII.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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DNA Restriction Enzymes*
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DNA*
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DNA, Fungal*
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DNA, Ribosomal
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Deoxyribonuclease BamHI
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Deoxyribonuclease HindIII
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Deoxyribonucleases, Type II Site-Specific*
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Electrophoresis, Agar Gel
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Nucleic Acid Hybridization
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Physarum / analysis*
Substances
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DNA, Fungal
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DNA, Ribosomal
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DNA
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DNA Restriction Enzymes
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Deoxyribonuclease BamHI
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Deoxyribonuclease HindIII
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Deoxyribonucleases, Type II Site-Specific
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GGTNACC-specific type II deoxyribonucleases