[Comparison between 2 density gradients for separation of CFU]

Biomedicine. 1980 Nov;33(7):236-9.
[Article in French]

Abstract

We separated 40 bone marrow samples by centrifugation on two density gradients, ficoll-metrizoate sodium (lymphoprep) and percoll. There is no difference between the two methods when expressing the colony forming cells (CFU-C) per 2.10(5) bone marrow cells plated. But the absolute number of recovered CFU-C is significantly greater with percoll than with ficoll metrizoate sodium.

Publication types

  • Comparative Study

MeSH terms

  • Bone Marrow Cells*
  • Cell Separation / methods*
  • Centrifugation, Density Gradient / methods*
  • Colony-Forming Units Assay
  • Ficoll*
  • Humans
  • Iodobenzoates*
  • Metrizoic Acid*
  • Polysaccharides*
  • Povidone*
  • Silicon Dioxide*

Substances

  • Iodobenzoates
  • Polysaccharides
  • Ficoll
  • Percoll
  • Silicon Dioxide
  • Metrizoic Acid
  • Povidone