2-5A synthetase activity does not increase in interferon-resistant Friend leukemia cell variants treated with alpha/beta interferon despite the presence of high-affinity interferon receptor sites

Virology. 1983 Mar;125(2):508-12. doi: 10.1016/0042-6822(83)90224-6.

Abstract

The presence of interferon (IFN) receptors on mouse Friend leukemia cells (FLC) has been investigated in binding experiments with highly purified 125I-labeled mouse alpha/beta IFN. Both IFN-resistant clones and wild-type IFN-sensitive FLC showed a specific saturable binding site for mouse IFN with a similar affinity constant. In contrast to IFN-sensitive FLC, IFN-resistant FLC variants were not inducible by IFN for double-stranded RNA-dependent 2-5A synthetase activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 2',5'-Oligoadenylate Synthetase / metabolism*
  • Animals
  • Clone Cells / metabolism
  • Friend murine leukemia virus
  • Interferon Type I / pharmacology*
  • Leukemia, Experimental / enzymology*
  • Mice
  • Receptors, Cell Surface / metabolism*
  • Receptors, Interferon

Substances

  • Interferon Type I
  • Receptors, Cell Surface
  • Receptors, Interferon
  • 2',5'-Oligoadenylate Synthetase