Expression of a microinjected immunoglobulin gene in the spleen of transgenic mice

Nature. 1983 Nov;306(5941):332-6. doi: 10.1038/306332a0.

Abstract

Transgenic mice were produced by microinjection of a rearranged, functional immunoglobulin kappa gene into fertilized mouse eggs and implantation of the microinjected embryos into foster mothers. Mice that integrated the injected gene were mated and the DNA, RNA and serum kappa chains of their offspring were analysed. The data from offspring of three different transgenic mice indicate that the microinjected gene is expressed in the spleen, but not the liver of mice which inherited the injected gene.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Gene Expression Regulation
  • Genes
  • Genetic Engineering
  • Immunoglobulin Light Chains / genetics*
  • Immunoglobulin kappa-Chains / genetics*
  • Isoelectric Point
  • Mice
  • Microinjections
  • Molecular Weight
  • RNA, Messenger / genetics
  • Spleen / immunology
  • Spleen / metabolism*
  • Transcription, Genetic

Substances

  • Immunoglobulin Light Chains
  • Immunoglobulin kappa-Chains
  • RNA, Messenger