Abstract
A spontaneously occurring, noninducible, chloramphenicol-resistant mutant of Bacillus subtilis 168 has a mutation (cam-2) which maps in the ribosomal protein region of the chromosome near dal. Its presence does not confer dependence on chloramphenicol. Ribosomes of the cam-2 strain remained sensitive to chloramphenicol in in vitro protein synthesis. No chloramphenicol acetyltransferase activity could be detected.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Acetyltransferases / metabolism
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Bacillus subtilis / drug effects
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Bacillus subtilis / enzymology
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Bacillus subtilis / genetics*
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Bacterial Proteins / analysis
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Chloramphenicol / pharmacology*
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Chloramphenicol O-Acetyltransferase
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Chromosome Mapping
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Chromosomes, Bacterial*
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Drug Resistance, Microbial
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Genes, Bacterial*
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Mutation
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Ribosomal Proteins / analysis
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Ribosomes / drug effects
Substances
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Bacterial Proteins
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Ribosomal Proteins
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Chloramphenicol
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Acetyltransferases
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Chloramphenicol O-Acetyltransferase