Abstract
Microinjection of purified pp60v-src into Xenopus oocytes caused the phosphorylation of ribosomal protein S6 on serine residues and also increased total protein phosphorylation, with almost a two-fold increase in the percentage of phosphotyrosine present. In addition, pp60v-src accelerated the time course of progesterone-induced oocyte maturation, suggesting that the biochemical pathway influenced by pp60v-src is related to that induced by progesterone.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acids / analysis
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Animals
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Electrophoresis, Polyacrylamide Gel
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Female
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Meiosis*
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Microinjections
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Oncogene Protein pp60(v-src)
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Oocytes / cytology
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Oocytes / drug effects
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Oocytes / metabolism*
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Phosphorylation
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Progesterone / pharmacology
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Protein Kinases / metabolism*
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Ribosomal Protein S6
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Ribosomal Proteins / isolation & purification
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Ribosomal Proteins / metabolism*
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Ribosomes / metabolism
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Viral Proteins / metabolism*
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Xenopus
Substances
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Amino Acids
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Ribosomal Protein S6
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Ribosomal Proteins
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Viral Proteins
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Progesterone
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Protein Kinases
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Oncogene Protein pp60(v-src)