The L-aspartate oxidase reported to be present in higher plants is actually glutamic oxaloacetic transaminase

J P Wilder; J A Sae-Lee; E D Mitchell; R K Gholson

doi:10.1016/0006-291x(84)90306-1

The L-aspartate oxidase reported to be present in higher plants is actually glutamic oxaloacetic transaminase

Biochem Biophys Res Commun. 1984 Sep 17;123(2):836-41. doi: 10.1016/0006-291x(84)90306-1.

Authors

J P Wilder, J A Sae-Lee, E D Mitchell, R K Gholson

PMID: 6487317
DOI: 10.1016/0006-291x(84)90306-1

Abstract

We previously reported (Biochem. Biophys. Res. Commun. (1983) 111, 188-193) that cotton callus cells contain an L-aspartate oxidase which requires an unidentified cofactor with an apparent molecular weight of 1,050. Further study has revealed that this report was in error. The enzyme is actually glutamic oxaloacetic transaminase and the "cofactor" has been identified as alpha-ketoglutarate.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Amino Acid Oxidoreductases / analysis*
Aspartate Aminotransferases / analysis*
Chromatography, Gel
Escherichia coli Proteins
Ketoglutaric Acids / metabolism
Molecular Weight
Oxaloacetates / metabolism
Plants / enzymology*

Substances

Escherichia coli Proteins
Ketoglutaric Acids
Oxaloacetates
Amino Acid Oxidoreductases
L-aspartate oxidase, E coli
Aspartate Aminotransferases