A simple device to obtain high local concentrations of material sorted by flow cytometry for biochemical or morphological analysis

Cytometry. 1984 Sep;5(5):550-2. doi: 10.1002/cyto.990050520.

Abstract

A system is described which allows us to collect flow cytometry sorted objects onto a very small area, thus obtaining very concentrated particles. For that purpose, collection is made directly on a nitrocellulose filter while a vacuum is established below the filter. By comparison with collection onto a microscope slide, the local concentration is increased about 1,000-fold. This efficient and simple system allows us to concentrate cell or cellular organelle populations for observation by microscopy of many objects in a single field, or to study the biological or biochemical properties of a small number of sorted particles.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Collodion
  • Flow Cytometry / instrumentation*
  • Flow Cytometry / methods
  • Micropore Filters
  • Vacuum

Substances

  • Collodion