Inexact three-dimensional reconstruction of a biological macromolecule from a restricted number of projections

Ultramicroscopy. 1984;13(4):365-71. doi: 10.1016/0304-3991(84)90002-0.

Abstract

A computer-generated three-dimensional model of Lumbricus terrestris hemoglobin has been obtained by reconstruction from four STEM low-dose computer-averaged micrographs. X-ray projections of a wooden model constructed from the calculated two-dimensional sections through the molecule were generally consistent with the original STEM projections of the hemoglobin and suggest the presence of large gaps between the central regions of adjacent 1/12th subunits of opposite tiers.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Annelida / analysis
  • Computers
  • Hemoglobins*
  • Methods
  • Models, Molecular*

Substances

  • Hemoglobins