Estimates of validity of projection approximation for three-dimensional reconstructions at high resolution

Ultramicroscopy. 1984;14(3):219-26. doi: 10.1016/0304-3991(84)90090-1.

Abstract

As spatial frequency increases, the electron microscope "image" deviates increasingly from a true projection of the specimen's structure. This is due to the finite radius of the Ewald sphere. Quantitative estimates of these deviations of the reconstruction from the true projection are presented for a range of accelerating voltages, spatial frequencies, specimen thicknesses, and specimen tilts.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Data Display*
  • Microscopy, Electron / standards*