Reference values are essential for interpretation of biochemical data on patients. Due to various problems, such values could not be obtained for the local paediatric population. This study was carried out to establish reference values for 24 biochemical constituents using blood specimens obtained from 352 Singapore children who were assessed to be free from any disease that may affect the values. For a number of biochemical constituents, reference values were also calculated for the different age groups. These values are compared with the local adult values established by the same methods.