426 strains recent clinically isolates have been investigated with respect to their sensitivity against several chemioantibiotics. Data obtained with the Bauer and Kirby standard agar diffusion method have been compared with those carried out by using a new computerized system (Cobas-Bact, Roche), which is able to perform antibiograms in five hours. The performance of Cobas-Bact has been very significative, since the overall major concordance between the two methods was higher than 94%.