Abstract
Transducin was [32P]ADP-ribosylated by cholera toxin in bovine retinal rod outer segments and then partially purified on omega-amino octyl agarose to remove other ADP-ribosylated proteins. Trypsin digestion of the ADP-ribosylated transducin and further purification using boronate-polyacrylamide beads and high performance liquid chromatography yielded a single radiolabeled tetrapeptide, Ser-Arg-Val-Lys. The ADP-ribose is linked to the guanidinium group of arginine.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenosine Diphosphate Ribose / metabolism*
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Amino Acid Sequence
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Animals
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Cattle
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Cholera Toxin / pharmacology*
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Chromatography, High Pressure Liquid
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Membrane Proteins / analysis*
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Membrane Proteins / metabolism
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Nucleoside Diphosphate Sugars / metabolism*
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Photoreceptor Cells / analysis*
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Rod Cell Outer Segment / analysis*
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Transducin
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Trypsin / metabolism
Substances
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Membrane Proteins
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Nucleoside Diphosphate Sugars
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Adenosine Diphosphate Ribose
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Cholera Toxin
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Trypsin
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Transducin