Electron microscopic evidence for the axial rotation and inter-domain flexibility of the Fab regions of immunoglobulin G

J Mol Biol. 1983 Sep 25;169(3):771-4. doi: 10.1016/s0022-2836(83)80170-3.

Abstract

The Fab arms of immunoglobulin G (IgG) have long been known to hinge about their joint with the Fc subunit. Using monoclonal antibodies bound to influenza haemagglutinin (HA) as position markers, we now show that these arms can also rotate about their long axis with respect to Fc. We also show that when two IgGs are bound cyclically with two HA molecules, the arms can bend between the variable and constant domains to accommodate bond angle constraint.

MeSH terms

  • Antigen-Antibody Complex
  • Hemagglutinins, Viral
  • Immunoglobulin Fab Fragments*
  • Immunoglobulin G*
  • Microscopy, Electron
  • Rotation

Substances

  • Antigen-Antibody Complex
  • Hemagglutinins, Viral
  • Immunoglobulin Fab Fragments
  • Immunoglobulin G