[Molecular genetic analysis of a cold-adapted donor attenuation strain for a pediatric liver influenza vaccine, of recombinants based on it and of isolates from vaccinated children]

Vopr Virusol. 1984 Jan-Feb;29(1):22-8.
[Article in Russian]

Abstract

The recombination test demonstrated that in the genome of a highly attenuated cold-adapted variant of influenza A/Leningrad/134/47/57 virus five genes coding for nonglycosylated proteins (1, 2, 5, 7, 8) carried ts mutations. Hybridization of this variant with epidemically important strains of influenza H1N1 and H3N2 viruses produced recombinants which inherited hemagglutinin and neuraminidase from the epidemic strains and 5 (H1N1) and 6 (H3N2) genes coding for nonglycosylated proteins from the cold-adapted variant. The genomes of both recombinants were found to contain five genes coding for nonglycosylated proteins which carried ts mutations. High genetic stability of cold-adapted recombinant vaccine strains was established in immunized children.

Publication types

  • Comparative Study
  • English Abstract

MeSH terms

  • Adaptation, Physiological*
  • Child
  • Cold Temperature*
  • Genes, Viral
  • Humans
  • Influenza A virus / genetics*
  • Influenza A virus / immunology
  • Influenza A virus / isolation & purification
  • Influenza Vaccines / analysis*
  • Influenza Vaccines / immunology
  • Influenza Vaccines / isolation & purification
  • Mutation
  • Nucleic Acid Hybridization
  • Phenotype
  • RNA, Viral / genetics
  • Recombination, Genetic*
  • Temperature
  • Vaccines, Attenuated / analysis
  • Vaccines, Attenuated / immunology
  • Vaccines, Attenuated / isolation & purification

Substances

  • Influenza Vaccines
  • RNA, Viral
  • Vaccines, Attenuated