Abstract
Nicotinamide nucleotide transhydrogenase in rat liver submitochondrial particles is inhibited by treatment with NN'-dicyclohexylcarbodi-imide or butane-2,3-dione. Both inhibitions are pseudo-first-order with respect to enzyme activity. The reaction order with respect to inhibitor is close to unity for butanedione, but is significantly lower than unity for dicyclohexylcarbodi-imide.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Butanones / pharmacology*
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Carbodiimides / pharmacology*
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Diacetyl / pharmacology*
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Dicyclohexylcarbodiimide / pharmacology*
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In Vitro Techniques
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Kinetics
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Male
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Mitochondria, Liver / drug effects
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Mitochondria, Liver / enzymology*
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NADH, NADPH Oxidoreductases / antagonists & inhibitors*
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NADP Transhydrogenases / antagonists & inhibitors*
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Rats
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Rats, Inbred Strains
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Submitochondrial Particles / drug effects
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Submitochondrial Particles / enzymology
Substances
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Butanones
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Carbodiimides
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Dicyclohexylcarbodiimide
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NADH, NADPH Oxidoreductases
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NADP Transhydrogenases
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Diacetyl