The induction of mitotic gene conversion in the yeast, Saccharomyces cerevisiae JD1 by 4-chloromethylbiphenyl (4CMB), benzyl chloride (BC) and 4-hydroxymethylbiphenyl (4HMB)

Mutat Res. 1982 Jan-Feb;100(1-4):157-62. doi: 10.1016/0165-1218(82)90038-6.

Abstract

The induction of mitotic gene conversion by 4CMB, BC and 4HMB was studied in both log-phase and stationary-phase cultures of the yeast, Saccharomyces cerevisiae JD1. Assays were performed both in the presence and in the absence of S9 microsomal fraction obtained from a liver homogenate from rats pretreated with Aroclor 1254. Exposure of both stationary-phase and log-phase cultures to 4CMB and BC resulted in an increase in mitotic gene conversion, both in the presence and in the absence of a microsomal activation system; the magnitude of response was greater in stationary-phase cultures. 4HMB did not increase the gene conversion frequency in log-phase or stationary-phase cultures.

MeSH terms

  • Animals
  • Benzyl Compounds / pharmacology*
  • Biotransformation
  • Biphenyl Compounds / pharmacology*
  • Gene Conversion*
  • Microsomes, Liver / metabolism
  • Mitosis
  • Mutagenicity Tests
  • Mutagens / pharmacology*
  • Mutation*
  • Rats
  • Saccharomyces cerevisiae / drug effects
  • Saccharomyces cerevisiae / genetics*

Substances

  • Benzyl Compounds
  • Biphenyl Compounds
  • Mutagens
  • 4-hydroxymethylbiphenyl
  • 4-chloromethylbiphenyl
  • benzyl chloride