This investigation of the junctional pattern of the human trophoblastic epithelium was carried out on the fractured faces of (a) the syncytial plasma membrane bathed by the maternal blood (S-PMm); (b) by syncytial plasma membrane in contact with either the cytotrophoblast or the trophoblastic basement membrane (S-PMf), and (c) the cytotrophoblastic plasma membrane (C-PM). The most relevant findings were tight junctions on the SPMm, and the intertrophoblastic junctional pattern, not realizing a tight subcompartmentalization between syncytium and cytotrophoblast. The following aspects were discussed: the SPMf represents the last membrane barrier before the fetal capillary, and the function of the cytotrophoblast seems to be limited to the modulation of syncytial activity.