Specific immunoadsorption of IgG antibody in a patient with chronic lymphocytic leukemia and autoimmune hemolytic anemia. A new form of therapy for the acute critical stage

Am J Med. 1981 Dec;71(6):1035-40. doi: 10.1016/0002-9343(81)90337-5.

Abstract

Progressive and severe autoimmune hemolytic anemia developed in a patient with chronic lymphocytic leukemia (CLL) despite treatment with chlorambucil, high doses of corticosteroids and attempts to transfuse packed red blood cells. Splenectomy was not performed because of severe coronary artery disease. Direct antiglobulin tests revealed a warm red blood cell autoantibody of IgG-type with anti-e specificity. The patient was treated by extracorporeal immunoadsorption of plasma IgG using a cell separator and protein A as the immunoadsorbent. The patient responded by an increase in the hemoglobin levels and platelet counts after two treatments. Specificity of the procedure was shown by a decrease in the serum IgG and by the demonstration of the same reactivity to ficin-treated reagent red blood cell panel of the eluate from the protein A. Antibody titers of the patient's red blood cell eluate decreased from 1:128 to 1:64 and eventually the anti-e specificity was lost. This is a report of a novel approach to treatment of the acute phase of an autoimmune hemolytic anemia.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adsorption
  • Aged
  • Anemia, Hemolytic, Autoimmune / complications
  • Anemia, Hemolytic, Autoimmune / therapy*
  • Antibody Specificity
  • Cell Separation
  • Chlorambucil / therapeutic use
  • Hemoglobins / analysis
  • Humans
  • Immunoglobulin G*
  • Leukapheresis
  • Leukemia, Lymphoid / complications
  • Leukemia, Lymphoid / therapy*
  • Male
  • Platelet Count
  • Prednisone / therapeutic use
  • Staphylococcal Protein A

Substances

  • Hemoglobins
  • Immunoglobulin G
  • Staphylococcal Protein A
  • Chlorambucil
  • Prednisone