B-50 (GAP-43) in the rat spinal cord caudal to hemisection: lack of intraspinal sprouting by dorsal root axons

W Nacimiento; T Sappok; G A Brook; L Tóth; A B Oestreicher; W H Gispen; J Noth; G W Kreutzberg

doi:10.1016/0304-3940(95)11707-4

B-50 (GAP-43) in the rat spinal cord caudal to hemisection: lack of intraspinal sprouting by dorsal root axons

Neurosci Lett. 1995 Jul 14;194(1-2):13-6. doi: 10.1016/0304-3940(95)11707-4.

Authors

W Nacimiento¹, T Sappok, G A Brook, L Tóth, A B Oestreicher, W H Gispen, J Noth, G W Kreutzberg

Affiliation

¹ Department of Neurology, Aachen University Medical School, Germany.

PMID: 7478195
DOI: 10.1016/0304-3940(95)11707-4

Abstract

The controversial hypothesis that intraspinal sprouting by dorsal root axons promotes reinnervation of partially denervated neurons caudal to a low thoracic cord hemisection was re-investigated in rats using quantitative immunohistochemical analysis of the neural specific growth-associated protein B-50 (GAP-43) at postoperative survival times of 3, 10, 21, 42, and 90 days. The lack of increase in B-50-immunoreactivity in all segments below the hemisection at all survival times does not support the concept of intraspinal sprouting following the removal of supraspinal descending pathways.

MeSH terms

Animals
Axons / metabolism
Female
GAP-43 Protein
Ganglia, Spinal / immunology
Ganglia, Spinal / metabolism*
Immunohistochemistry
Membrane Glycoproteins / biosynthesis*
Membrane Glycoproteins / metabolism*
Nerve Degeneration
Nerve Tissue Proteins / biosynthesis*
Nerve Tissue Proteins / metabolism*
Neuronal Plasticity / physiology
Phosphoproteins / biosynthesis*
Phosphoproteins / metabolism*
Pyramidal Cells / immunology
Rats
Rats, Inbred Strains
Spinal Cord / immunology
Spinal Cord / metabolism*
Spinal Cord Injuries
Time Factors

Substances

GAP-43 Protein
Membrane Glycoproteins
Nerve Tissue Proteins
Phosphoproteins