Abstract
Purpose:
To test whether a nontargeted, long-circulating, synthetic polymer accumulates in areas of inflammation, with high capillary permeability and increased regional blood flow.
Materials and methods:
Methoxy poly(ethylene glycol)-poly-L-lysine (PL)-diethylenetriaminepentaacetic acid (MPEG-PL-DTPA) was labeled with technetium-99m for scintigraphy and with gadolinium for magnetic resonance (MR) imaging. Eleven Escherichia coli-infected rats were injected with 1.0 mCi (37 MBq) of Tc-99m-labeled MPEG-PL-DTPA for scintigraphy. Twelve rats underwent 1.5-T MR imaging after intravenous injection of gadolinium-labeled MPEG-PL-DTPA (35 mumol/kg).
Results:
Tc-99m-labeled MPEG-PL-DTPA demonstrated nearly eight-fold higher accumulation in infected muscle when compared with normal muscle. Scintigrams and MR images showed areas of inflammation with peak accumulation at 24 hours after injection of Tc-99m- or gadolinium-labeled MPEG-PL-DTPA.
Conclusion:
Nontargeted, long-circulating, copolymers can efficiently accumulate in sites of inflammation and thus represent an alternative to inflammation-specific agents.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Capillary Permeability
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Contrast Media* / pharmacokinetics
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Disease Models, Animal
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Escherichia coli Infections / diagnosis*
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Escherichia coli Infections / diagnostic imaging
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Escherichia coli Infections / metabolism
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Escherichia coli Infections / pathology
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Feasibility Studies
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Gadolinium DTPA*
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Gadolinium* / pharmacokinetics
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Immunoglobulin G
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Indium Radioisotopes
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Injections, Intravenous
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Magnetic Resonance Imaging
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Male
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Muscle, Skeletal / blood supply
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Muscle, Skeletal / diagnostic imaging
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Muscle, Skeletal / metabolism
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Muscle, Skeletal / microbiology
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Pentetic Acid / analogs & derivatives*
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Pentetic Acid / pharmacokinetics
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Polyethylene Glycols* / pharmacokinetics
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Polylysine / analogs & derivatives*
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Polylysine / pharmacokinetics
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Radionuclide Imaging
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Rats
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Rats, Sprague-Dawley
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Regional Blood Flow
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Soft Tissue Infections / diagnosis*
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Soft Tissue Infections / diagnostic imaging
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Soft Tissue Infections / metabolism
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Soft Tissue Infections / pathology
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Technetium Tc 99m Pentetate* / pharmacokinetics
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Thigh
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Time Factors
Substances
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Contrast Media
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Immunoglobulin G
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Indium Radioisotopes
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monomethoxypolyethylene glycol-polylysine-gadolinium DTPA
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Polylysine
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Polyethylene Glycols
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Pentetic Acid
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Gadolinium
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Gadolinium DTPA
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Technetium Tc 99m Pentetate