The mouse B-cell repertoire early in ontogeny contains B cells with receptor immunoglobulins exhibiting high connectivity, multi/self-reactivity, and generally low affinity. This is due structurally to extensive restriction in the germline components used to generate the B-cell receptor. The selective pressure acting on the nascent repetoire has both negative and positive components as seen in our in vivo models. VH81X-bearing B cells from the VH81X transgenic mice (and probably from normal mice) are subject to self-selective pressure with two components: a positive one favoring a certain (self-reactive) specificity in the CD23-IgM+ population and a negative one preventing the entry of B cells with this specificity into the CD23+IgM+ compartment.