Unidirectional cross-phosphorylation between the granulocyte colony-stimulating factor and interleukin 3 receptors

J Biol Chem. 1993 Dec 5;268(34):25818-23.

Abstract

The mouse interleukin-3 (IL-3)-dependent hemopoietic precursor cell line 32DC13 responds to granulocyte colony-stimulating factor (G-CSF) for proliferation and differentiation. We established a subline (32D-FH) of the 32DC13 cells which has lost the ability to respond to G-CSF. When murine G-CSF receptor cDNA was introduced into the 32D-FH cell line, the transformants responded to G-CSF as well as to IL-3 for proliferation. Adding G-CSF to the transformants rapidly induced tyrosine phosphorylation not only of the G-CSF receptor but also of both subtypes of the IL-3 receptor beta-chain (AIC2A and AIC2B molecules). On the other hand, stimulation of the transformants with IL-3 induced tyrosine phosphorylation of the AIC2A and AIC2B but not of the G-CSF receptor. These results indicate that the tyrosine kinase activated through the G-CSF receptor interacts with the IL-3 receptor beta-chains but not vice versa. This unidirectional cross-phosphorylation between the G-CSF and IL-3 receptors may play a role in granulopoiesis induced by G-CSF.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acids / analysis
  • Animals
  • Cell Differentiation / drug effects
  • Cell Division / drug effects
  • Cell Line
  • Granulocyte Colony-Stimulating Factor / pharmacology*
  • Hematopoietic Stem Cells / cytology
  • Hematopoietic Stem Cells / drug effects
  • Hematopoietic Stem Cells / metabolism
  • Humans
  • Interleukin-3 / pharmacology*
  • Kinetics
  • Macromolecular Substances
  • Mice
  • Phosphorylation
  • Phosphoserine / analysis
  • Phosphothreonine / analysis
  • Phosphotyrosine
  • Receptor Protein-Tyrosine Kinases / metabolism*
  • Receptors, Granulocyte Colony-Stimulating Factor / biosynthesis
  • Receptors, Granulocyte Colony-Stimulating Factor / metabolism*
  • Receptors, Interleukin-3 / biosynthesis
  • Receptors, Interleukin-3 / metabolism*
  • Recombinant Proteins / pharmacology
  • Transfection
  • Tyrosine / analogs & derivatives
  • Tyrosine / analysis

Substances

  • Amino Acids
  • Interleukin-3
  • Macromolecular Substances
  • Receptors, Granulocyte Colony-Stimulating Factor
  • Receptors, Interleukin-3
  • Recombinant Proteins
  • Phosphothreonine
  • Granulocyte Colony-Stimulating Factor
  • Phosphoserine
  • Phosphotyrosine
  • Tyrosine
  • Receptor Protein-Tyrosine Kinases