Abstract
The potential neurotrophic effect of substance P-like immunoreactivity present in culture media was assessed in rat embryonic day 18 hippocampal cultures. The neurokinin-1 (substance P) receptor antagonist CP-96345 induced neurotoxicity that was dose dependent and attenuated by addition of substance P or the neurokinin-1 agonist [Sar9,Met(O2)11]-SP. These studies suggest that under some conditions neurokinin-1 receptor stimulation promotes neuronal survival.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Biphenyl Compounds / pharmacology
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Biphenyl Compounds / toxicity
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Cells, Cultured
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Culture Media, Conditioned
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Hippocampus / cytology*
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Hippocampus / drug effects
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Neurokinin-1 Receptor Antagonists
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Neurons / drug effects*
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Polymerase Chain Reaction
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RNA, Messenger / biosynthesis
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Rats
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Receptors, Neurokinin-1 / drug effects
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Substance P / pharmacology*
Substances
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Biphenyl Compounds
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Culture Media, Conditioned
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Neurokinin-1 Receptor Antagonists
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RNA, Messenger
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Receptors, Neurokinin-1
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Substance P
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CP 96345