Distribution of integrin subunits in normal human kidney

Cell Adhes Commun. 1994 Jun;2(2):159-67. doi: 10.3109/15419069409004434.

Abstract

We evaluated on serial sections the distribution of a large number of integrin alpha and beta chains in normal adult human kidney: 1) the beta 1 chain and its corresponding alpha subunits (alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, alpha 6), 2) alpha v and beta 3 chains, 3) the beta 2 chain and its corresponding alpha chains (alpha X, alpha M, alpha L), and 4) the beta 4 chain. We also evaluated ICAM-1, VCAM and ELAM and the major extracellular matrix components (ECM). A three step immunoperoxidase technique was used on frozen sections. Each cell of the kidney shows a specific distribution of these molecules. The relation with ECM and some of their ligands was evaluated. This immunohistochemical study shows that there is no strict colocalisation of a given ECM component with its specific receptor.

MeSH terms

  • Adult
  • Antibodies, Monoclonal
  • Basement Membrane / metabolism
  • Cell Adhesion Molecules / metabolism
  • E-Selectin
  • Extracellular Matrix / metabolism
  • Humans
  • Immunoenzyme Techniques
  • Integrins / chemistry
  • Integrins / immunology
  • Integrins / metabolism*
  • Intercellular Adhesion Molecule-1
  • Kidney / metabolism*
  • Kidney Glomerulus / metabolism
  • Kidney Tubules / metabolism
  • Methotrexate / metabolism
  • Protein Conformation
  • Tissue Distribution
  • Vascular Cell Adhesion Molecule-1 / metabolism

Substances

  • Antibodies, Monoclonal
  • Cell Adhesion Molecules
  • E-Selectin
  • Integrins
  • Vascular Cell Adhesion Molecule-1
  • Intercellular Adhesion Molecule-1
  • Methotrexate