Evaluation of third-generation assays for detection of anti-hepatitis C virus (HCV) antibodies and comparison with presence of HCV RNA in blood donors reactive to c100-3 antigen

D Lavanchy; C Mayerat; B Morel; P Schneider; C Zufferey; J J Gonvers; A Pécoud; P C Frei

doi:10.1128/jcm.32.9.2272-2275.1994

Evaluation of third-generation assays for detection of anti-hepatitis C virus (HCV) antibodies and comparison with presence of HCV RNA in blood donors reactive to c100-3 antigen

J Clin Microbiol. 1994 Sep;32(9):2272-5. doi: 10.1128/jcm.32.9.2272-2275.1994.

Authors

D Lavanchy¹, C Mayerat, B Morel, P Schneider, C Zufferey, J J Gonvers, A Pécoud, P C Frei

Affiliation

¹ Division of Immunology and Allergy, Centre Hospitalier Universitaire Vaudois, Lausanne, Switzerland.

Abstract

We tested serum samples from blood donors by using first-, second-, and third-generation enzyme immunoassays or recombinant immunoblot assays. Second- and third-generation assays gave comparable results. Circulating hepatitis C virus RNA was found in a high proportion of reactive samples. A lack of reactivity or low-level reactivity predicted the absence of hepatitis C virus RNA in 100% of the cases.

Publication types

Comparative Study

MeSH terms

Antigens, Viral*
Blood Donors*
Evaluation Studies as Topic
Hepacivirus / genetics
Hepacivirus / immunology
Hepacivirus / isolation & purification*
Hepatitis Antibodies / blood*
Hepatitis C / diagnosis
Hepatitis C / epidemiology
Hepatitis C / prevention & control*
Hepatitis C Antibodies
Humans
Immunoblotting*
Immunoenzyme Techniques*
Predictive Value of Tests
RNA, Viral / blood*
Reagent Kits, Diagnostic*
Viral Nonstructural Proteins / immunology*

Substances

Antigens, Viral
C100 protein, hepatitis C virus
Hepatitis Antibodies
Hepatitis C Antibodies
RNA, Viral
Reagent Kits, Diagnostic
Viral Nonstructural Proteins