Abstract
An improved method for the preparation of a double-stranded RNA-coupled resin is described. Periodate-oxidized double-stranded RNA, in the form of either purified reovirus double-stranded RNA or poly(rl). poly(rC), was covalently attached to agarose-adipic acid hydrazide. The resulting affinity resin had increased specificity for known double-stranded RNA binding proteins, as compared to a similar commercially available resin. The application of this resin in the purification and characterization of double-stranded RNA binding proteins is described.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Binding, Competitive
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Capsid Proteins*
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Cells, Cultured
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Chromatography, Affinity / methods*
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Electrophoresis, Polyacrylamide Gel
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Interferons / pharmacology
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Protein Kinases / isolation & purification
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RNA, Double-Stranded / chemistry*
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RNA, Double-Stranded / genetics
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RNA, Double-Stranded / metabolism
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RNA-Binding Proteins / isolation & purification*
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RNA-Binding Proteins / metabolism
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Reoviridae / genetics
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Sepharose / chemistry*
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Viral Proteins / isolation & purification
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Viral Proteins / metabolism
Substances
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Capsid Proteins
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RNA, Double-Stranded
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RNA-Binding Proteins
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Viral Proteins
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sigma protein 3, Reovirus
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Interferons
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Sepharose
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Protein Kinases