Complete removal of mycoplasma from viral preparations using solvent extraction

J Virol Methods. 1995 Mar;52(1-2):51-4. doi: 10.1016/0166-0934(94)00136-5.

Abstract

Mycoplasma contamination of two alphavirus isolates and adenovirus 5 was completely removed using a very simple, rapid protocol based on solvent extraction. Treated virus preparations remained free of mycoplasma after 6 months in culture.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoviruses, Human / isolation & purification
  • Adenoviruses, Human / physiology*
  • Bacteriological Techniques
  • Bisbenzimidazole
  • HeLa Cells
  • Humans
  • Mycoplasma / isolation & purification*
  • Ross River virus / isolation & purification
  • Ross River virus / physiology*
  • Solvents
  • Virology / methods

Substances

  • Solvents
  • Bisbenzimidazole