Enhanced production and gene expression of interleukin-5 in patients with bronchial asthma: possible management of atopic diseases by down-regulation of interleukin-5 gene transcription

Int Arch Allergy Immunol. 1995 May-Jun;107(1-3):255-8. doi: 10.1159/000236995.

Abstract

Interleukin (IL)-5 was produced in vitro by peripheral blood mononuclear cells (PBMCs) of mite-sensitive atopic patients upon challenge with specific allergen, while PBMCs of healthy controls produced essentially no IL-5. Stimuli delivered by the combination of phorbol ester (PMA) and CA2+ ionophore (ionomycin) induced marked IL-5 production by PBMCs obtained from atopic as well as nonatopic asthmatics. CD2- or CD4-bearing-cell depletion almost completely removed IL-5 production and gene transcription while CD8 depletion did not. These findings indicated that CD4+ T cells are the principal source of IL-5 in asthmatic PBMCs. The capacity of PBMCs of atopic asthmatics, nonatopic asthmatics and healthy controls to produce IL-2, IL-3, IL-4, interferon-gamma and granulocyte/macrophage-colony-stimulating factor was comparable among the three groups. FK506 suppressed IL-5 production and gene expression in vitro in a dose-dependent manner.

MeSH terms

  • Allergens
  • Animals
  • Antigens, Dermatophagoides
  • Asthma / genetics*
  • Asthma / metabolism
  • CD4-Positive T-Lymphocytes / drug effects
  • CD4-Positive T-Lymphocytes / metabolism*
  • DNA-Binding Proteins / physiology
  • Eosinophils / drug effects
  • Eosinophils / immunology
  • Glycoproteins
  • Granulocyte-Macrophage Colony-Stimulating Factor / biosynthesis
  • Granulocyte-Macrophage Colony-Stimulating Factor / genetics
  • Hypersensitivity, Immediate / genetics
  • Hypersensitivity, Immediate / therapy*
  • Interferon-gamma / biosynthesis
  • Interferon-gamma / genetics
  • Interleukin-5 / biosynthesis*
  • Interleukin-5 / genetics
  • Interleukins / biosynthesis
  • Interleukins / genetics
  • Ionomycin / pharmacology
  • Lymphocyte Activation / drug effects
  • Mites / immunology
  • NFATC Transcription Factors
  • Nuclear Proteins*
  • Tacrolimus / pharmacology*
  • Tetradecanoylphorbol Acetate / pharmacology
  • Transcription Factors / physiology
  • Transcription, Genetic / drug effects*

Substances

  • Allergens
  • Antigens, Dermatophagoides
  • DNA-Binding Proteins
  • Glycoproteins
  • Interleukin-5
  • Interleukins
  • NFATC Transcription Factors
  • Nuclear Proteins
  • Transcription Factors
  • Ionomycin
  • Interferon-gamma
  • Granulocyte-Macrophage Colony-Stimulating Factor
  • Tetradecanoylphorbol Acetate
  • Tacrolimus