Abstract
Using an in vitro model for the development of IFN-gamma-producing (Th1) and IL-4-producing (Th2) cells from CD4+ T lymphocytes expressing a transgenic TCR, we show that IL-12 and IL-4 are the most potent stimuli for the differentiation of naive T cells to effector populations. When combinations of cytokines are present during T cell priming, the effect of IL-4 is dominant. Furthermore, differentiated Th1 cells can be converted into IL-4 producers by exposure to IL-4, but the Th2 phenotype is not reversible. The stability of Th2 populations may limit the ability to regulate Th2-dominant responses in pathologic situations.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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CD4-Positive T-Lymphocytes / cytology
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CD4-Positive T-Lymphocytes / immunology
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Cell Differentiation / drug effects
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Cell Differentiation / immunology*
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Cells, Cultured
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Interleukin-12 / pharmacology
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Interleukin-4 / pharmacology
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Lymphocyte Activation / drug effects
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Lymphocyte Activation / physiology*
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Mice
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Mice, Transgenic
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Receptors, Antigen, T-Cell / immunology
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Th1 Cells / cytology
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Th1 Cells / immunology*
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Th2 Cells / cytology
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Th2 Cells / immunology*
Substances
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Receptors, Antigen, T-Cell
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Interleukin-12
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Interleukin-4