Isolation of cDNA for a plasma membrane H(+)-ATPase from guard cells of Vicia faba L

N Nakajima; H Saji; M Aono; N Kondo

doi:10.1093/oxfordjournals.pcp.a078839

Isolation of cDNA for a plasma membrane H(+)-ATPase from guard cells of Vicia faba L

Plant Cell Physiol. 1995 Jul;36(5):919-24. doi: 10.1093/oxfordjournals.pcp.a078839.

Authors

N Nakajima¹, H Saji, M Aono, N Kondo

Affiliation

¹ National Institute for Environmental Studies, Tsukuba, Japan.

PMID: 7551588
DOI: 10.1093/oxfordjournals.pcp.a078839

Abstract

cDNA encoding the plasma membrane H(+)-ATPase of guard cells of Vicia faba L. was isolated. The clone encoded a 105-kDa polypeptide (956 amino acids) that was 79-85% identical in terms of amino acid sequence to other plant H(+)-ATPases. High levels of mRNA explain the high H(+)-ATPase activity of these plasma membranes.

MeSH terms

Amino Acid Sequence
Base Sequence
Blotting, Northern
Cell Membrane / enzymology*
Cloning, Molecular
DNA, Complementary / genetics
Fabaceae / enzymology
Fabaceae / genetics*
Molecular Sequence Data
Plant Leaves / cytology
Plant Leaves / enzymology*
Plants, Medicinal*
Proton-Translocating ATPases / genetics*
Sequence Analysis, DNA
Sequence Homology, Amino Acid

Substances

DNA, Complementary
Proton-Translocating ATPases

Associated data

GENBANK/S79323