Abstract
Three new cell lines of human glioblastoma have been established. These cells co-expressed hepatocyte growth factor (HGF) and its receptor, c-Met, genes in vitro. Reverse-transcriptase/polymerase-chain reaction study revealed that the cells also expressed gene for HGF activator, a recently cloned serine proteinase, suggesting that HGF might have a role in glioma cells in vitro as an autocrine factor. The activator mRNA was also detected in other well-established glioma cell lines, glioma tissues and normal brain. The concomitant expression of HGF, HGF activator and c-met was also detected in one glioblastoma case in vivo out of five tested.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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DNA Primers
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Gene Expression*
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Glioblastoma / genetics
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Glioblastoma / metabolism*
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Hepatocyte Growth Factor / genetics*
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Hepatocyte Growth Factor / metabolism
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Humans
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Male
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Middle Aged
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Molecular Sequence Data
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Proto-Oncogene Proteins c-met
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Receptor Protein-Tyrosine Kinases / genetics*
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Serine Endopeptidases / genetics*
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Tumor Cells, Cultured
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Urokinase-Type Plasminogen Activator / metabolism
Substances
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DNA Primers
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RNA, Messenger
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Hepatocyte Growth Factor
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Proto-Oncogene Proteins c-met
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Receptor Protein-Tyrosine Kinases
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HGF activator
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Serine Endopeptidases
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Urokinase-Type Plasminogen Activator